Table 3.

Kinetic parameters associated with the uptake of p-aminohippuric acid (PAH) in the presence of various acamprosate concentrations in OAT1 expressing HEK293 cells. Data are reported as mean ± SEM of replicates in three cell passages obtained at Lab. 2.

Substrate—PAH	0 (µM) Acamprosate	625 (µM) Acamprosate	1250 (µM) Acamprosate	25,000 (µM) Acamprosate
Km, app (µM)	5.6 ± 1.4	11.9 ± 3.2	17.9 ± 3.2	36.5 ± 7.4 *
Vmax, app (pmol∙cm−2∙min−1)	21.1 ± 1.1	23.4 ± 1.7ns	24.4 ± 1.3ns	27.6 ± 2.1ns
Km (µM)	7.7 ± 1.3
Vmax (pmol∙cm−2∙min−1)	23.2 ± 0.7
Ki (µM)	1229 ± 341

K_m and V_max values were compared using an ANOVA test, where means were significantly different for K_m values and not significantly different for V_max values. Values were compared to 0 (µM) acamprosate using a Dunnett’s post-test; * p < 0.05 and ns: not significant.