Genes differentially expressed between mature (ANT20 + ANT30 + ANT45) and immature (ANT5 + ANT10) pod stages. (a) Correspondence multivariate analyses based on all expressed genes in PMB0225 and PHA1037 along the different fruit developmental stages ANT5, ANT10, ANT20, ANT30, and ANT45. The variances absorbed by the two first factors (Dim1 and Dim2) at each plot are indicated in parentheses. Mature stages (ANT20, ANT30, and ANT45) are enclosed in red ovals. (b) Venn diagram of differentially expressed genes in PMB0225 and PHA1037 accessions during fruit development and maturation stages. The 2487 genes shared by both cultivars can be considered as candidate maturation genes. In addition, other 841 DE genes were exclusively found in PMB0225 and 1263 in PHA1037. (c) Hierarchical clustering of 2487 candidate maturation genes in PMB0255 and PHA1037 cultivars. The clusters of up- and down-regulated genes at maturation stages (ANT20, ANT30, and ANT45), as compared to the immature ones (ANT5 and ANT10), are clearly shown.