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. 2020 May 18;11:2041731420919334. doi: 10.1177/2041731420919334

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — (a) Representative immunofluorescence images and (b) quantification of fluorescence intensity (CellProfiler Software) comparing BMPR2 (Alexa488) expression between MSCs and ASCs in vitro (n = 3; *p < 0.001). Nuclei stained with DAPI. Scale bars = 200 µm. Expression of BMPR2 was observed to be greater in ASCs compared to MSCs. (c) Flow cytometric analysis of donor-matched MSC and ASC populations stained with BMPR2-APC. One representative isotype control is shown for each cell type for clarity. (d) Mean population fluorescent intensity shown graphically; ASCs showed significantly higher staining intensity for BMPR2 in comparison to MSCs, with less variation in mean expression observed (n = 6; *p < 0.01).