Figure 2.

Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements in primary cortical neurons cultures after the sequentially addition of oligomycin (1 µM), FCCP (2 µM), rotenone (300 nM) and antimycin (1 µM) following treat with Ru265 (50 µM) for 24 h (a and b) or 48 h (c and d). Pretreatment with Ru265 (50 µM) for 30 min preserves OCR and ECAR 2 h after 30 min of OGD (e and f). Bars show the mean ± SD of data representative of three separate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, two-way ANOVA followed by Bonferroni’s post hoc test.