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Effect of BAPTA-AM and PKA inhibitors on the expression of BDNF induced by 10 ng/ml TNF-α. Incubation of smooth muscle tissues for 24 h with the Ca2+ chelating agent BAPTA-AM or a PKA inhibitor both abolished the increase in BDNF expression induced by 10 ng/ml TNF-α. Data are presented as the mean ± the standard error of the mean. BDNF, brain derived neurotrophic factor; TNF-α, tumor necrosis factor-α; PKA, protein kinase A.
