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Effect of BAPTA-AM and PKA inhibitors on the secretion of BDNF induced by 10 ng/ml TNF-α. Incubation of smooth muscle tissues for 24 h with the Ca2+ chelating agent BAPTA-AM abolished the increase in BDNF secretion induced by 10 ng/ml TNF-α. BDNF secretion was not significantly affected by PKA inhibitor treatment. Data are presented as the mean ± the standard error of the mean. BDNF, brain derived neurotrophic factor; TNF-α, Tumor Necrosis Factor-α; PKA, protein kinase A.
