Table 1.
HPLC–MS/MS acquisition parameters used for the analysis of the 37 marker compounds in the extracts of Hypochaerislaevigata var. hipponensis.
| No. | Compounds | Retention Time (min) | Scan Type | Polarity or (ESI Mode) | Precursor Ion [M-H]− (m/z) | MS2Fragments or Product Ions (m/z) |
|---|---|---|---|---|---|---|
| 1 | Quinic acid | 1.13 | MRM | Negative | 190.95 | 85.3–93.3 |
| 2 | Malic acid | 1.23 | MRM | Negative | 133.00 | 115.2–71.3 |
| 3 | Fumaric acid | 1.48 | MRM | Negative | 115.00 | 71.4 |
| 4 | Gallic acid | 3.00 | MRM | Negative | 168.85 | 125.2–79.2 |
| 5 | Protocatechic acid | 4.93 | MRM | Negative | 152.95 | 108.3 |
| 6 | Pyrocatechol | 6.48 | MRM | Negative | 109.00 | 108.35–91.3 |
| 7 | Chlorogenic acid | 7.13 | MRM | Negative | 353.15 | 191.2 |
| 8 | 4-OH-Benzoic acid | 7.39 | MRM | Negative | 136.95 | 93.3–65.3 |
| 9 | Vanillic acid | 8.57 | MRM | Negative | 166.90 | 152.3–108.3 |
| 10 | Caffeic acid | 8.80 | MRM | Negative | 178.95 | 135.2–134.3 |
| 11 | Syringic acid | 9.02 | MRM | Negative | 196.95 | 182.2–167.3 |
| 12 | Vanillin | 10.87 | MRM | Negative | 151.00 | 1363–92.2 |
| 13 | Salicylic acid | 11.16 | MRM | Negative | 136.95 | 93.3–65.3 |
| 14 | p-Coumaric acid | 11.53 | MRM | Negative | 162.95 | 119.3–93.3 |
| 15 | Rutin | 12.61 | MRM | Negative | 609.05 | 300.1–271.1 |
| 16 | Ferulic acid | 12.62 | MRM | Negative | 192.95 | 178.3 |
| 17 | Sinapic acid | 12.66 | MRM | Negative | 222.95 | 208.3–149.2 |
| 18 | Hesperidin | 12.67 | MRM | Negative | 609 | 301.1 |
| 19 | Isoquercitrin | 13.42 | MRM | Negative | 463.00 | 300.1–271.1 |
| 20 | Rosmarinic acid | 14.54 | MRM | Negative | 359.00 | 161.2–197.2 |
| 21 | Nicotiflorin | 14.68 | MRM | Negative | 593.05 | 285.1–255.2 |
| 22 | α-Coumaric acid | 15.45 | MRM | Negative | 162.95 | 119.4–93.3 |
| 23 | Rhoifolin | 16.11 | MRM | Negative | 577.05 | 269.2–211.1 |
| 24 | Quercitrin | 16.41 | MRM | Negative | 447.15 | 301.1–255.1 |
| 25 | Apigetrin | 16.59 | MRM | Negative | 431.00 | 268.2–239.2 |
| 26 | Coumarin | 17.40 | MRM | Negative | 147.05 | 91.0–103.2 |
| 27 | Myricetin | 18.72 | MRM | Negative | 317.00 | 179.2–151.3 |
| 28 | Fisetin | 19.30 | MRM | Negative | 284.95 | 135.2–121.3 |
| 29 | Cinnamic acid | 25.61 | MRM | Negative | 147.00 | 103.15–77.3 |
| 30 | Liquiritigenin | 25.62 | MRM | Negative | 254.95 | 119.3–135.1 |
| 31 | Quercetin | 28.17 | MRM | Negative | 300.90 | 151.2–179.2 |
| 32 | Luteolin | 28.27 | MRM | Negative | 284.75 | 133.2–151.2 |
| 33 | Naringenin | 30.68 | MRM | Negative | 270.95 | 151.2–119.3 |
| 34 | Apigenin | 31.43 | MRM | Negative | 268.95 | 117.3–151.2 |
| 35 | Hesperetin | 31.76 | MRM | Negative | 300.95 | 164.2–136.2 |
| 36 | Kaempferol | 31.88 | MRM | Negative | 284.75 | 255.1–117.3 |
| 37 | Chrysin | 36.65 | MRM | Negative | 252.95 | 143.3–119.4 |