| Antibodies |
| β-Actin Antibody |
Cell Signaling |
Cat#4967; RRID: AB_330288 |
| Mouse Anti-PKC iota Monoclonal Antibody |
BD Biosciences |
Cat#610176; RRID: AB_397575 |
| Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) |
Cell Signaling |
Cat#5024; RRID: AB_1904142 |
| Sox2 Antibody |
Cell Signaling |
Cat#2748; RRID: AB_823640 |
| Anti-ECT2 Antibody |
Millipore Sigma |
Cat#07–1364; RRID: AB_10805932 |
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 |
Thermo Fisher Scientific |
Cat#A-11057; RRID: AB_2534104 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher Scientific |
Cat#A-21206; RRID: AB_2535792 |
| Goat Anti-P53 Polyclonal antibody |
Santa Cruz Biotechnology |
Cat#sc-6243-G; RRID: AB_653754 |
| p-SOX2 (T118) |
21st centry biochemicals |
PMID: 24525231 |
| p-ECT2 (T328) |
21st centry biochemicals |
PMID: 21189248 |
| Human p63/TP73L Antibody |
R&D systems |
Cat#AF1916; RRID:AB_2207174 |
| Cytokeratin 5 antibody |
Abcam |
Cat# ab52635; RRID:AB_869890 |
| Ki67 antibody |
Abcam |
Cat# ab15580; RRID:AB_443209 |
| normal rabbit IgG-B antibody |
Santa Cruz Biotechnology |
Cat#sc-2763; RRID: AB_737189 |
| Anti-Gli2 antibody |
Abcam |
Cat#ab26056; RRID:AB_2111901 |
| Anti-Wnt11 antibody |
Abcam |
Cat#ab31962; RRID:AB_883537 |
| Recombinant Anti-LEF1 antibody |
Abcam |
Cat#ab137872 |
| TROP2 / TACSTD2 Antibody (Extracellular Domain, CF) |
LifeSpan |
Cat#LS-C126415; RRID:AB_10833496 |
| Alexa Fluor® 647 anti-human/mouse CD49f Antibody |
BioLegend |
Cat#313610; RRID:AB_493637 |
| Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) |
BD Biosciences |
Cat#553142; RRID: AB_394657 |
| Biotin Rat Anti-Mouse CD31 |
BD Biosciences |
Cat#553371; RRID: AB_394817 |
| Biotin Mouse Anti-Mouse CD45.1 |
BD Biosciences |
Cat#553774; RRID: AB_395042 |
| Bacterial and Virus Strains |
|
E. coli DH5α |
Thermo Fisher Scientific |
Cat#18265017 |
| Ad-CMV-iCre |
Vector biolabs |
Cat#1045 |
| Ad-CMV-Null |
Vector biolabs |
Cat#1300 |
| Chemicals, Peptides, and Recombinant Proteins |
| TaqMan Fast Universal PCR Master Mix |
Thermo Fisher Scientific |
Cat#4352042 |
| PowerUp SYBR Green Master Mix |
Applied Biosystems |
Cat#A25778 |
| Protein G Mag Sepharose® Xtra |
GE Healthcare |
Cat#GE28–9670-70 |
| Pierce 16% Formaldehyde (w/v), Methanol-free |
Thermo Scientific |
Cat#28906 |
| Halt Protease Inhibitor Cocktail, EDTA-Free |
Thermo Scientific |
Cat#78437 |
| Pierce DTT (Dithiothreitol), No-Weigh Format |
Thermo Scientific |
Cat#A39255 |
| Micrococcal Nuclease |
New England Biolabs |
Cat#M0247S |
| RNase A, DNase and protease-free (10 mg/mL) |
Thermo Scientific |
Cat#EN0531 |
| Proteinase K Solution (20 mg/mL) |
Thermo Scientific |
Cat#AM2546 |
| SeaPlaque GTGTM Agarose |
Lonza |
Cat#50111 |
| Auranofin (ANF) |
Santa Cruz Biotechnology |
Cat#sc-202476A |
| GANT61 |
Selleckchem |
Cat#S8075 |
| LDE225 (Sonidegib, Erismodegib) |
Selleckchem |
Cat#S2151 |
| LDN-193189 |
Selleckchem |
Cat#S2618 |
| Dispase (1 U/mL) |
STEMCELL Technologies |
Cat#07923 |
| DNase I, RNase-free (1 U/μL) |
Thermo Fisher Scientific |
Cat#EN0521 |
| Bronchial Epithelial Cell Growth Medium (BEGM) |
Lonza |
Cat#CC-3170 |
| Matrigel Matrix |
CORNING |
Cat#354234 |
| Critical Commercial Assays |
| High-Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
Cat#4368813 |
| TURBO DNA-free Kit |
Thermo Fisher Scientific |
Cat#AM1907 |
| RNAqueous Total RNA Isolation Kit |
Thermo Fisher Scientific |
Cat#AM1912 |
| ViraPower lentiviral packaging mix |
Invitrogen |
Cat#K4975–00 |
| NucleoSpin® RNA Virus Kit |
Silica-membrane technology |
Cat#740956 |
| QIAprep Spin Miniprep Kit |
QIAGEN |
Cat#27106 |
| MinElute PCR Purification Kit |
QIAGEN |
Cat#28006 |
| QIAprep Spin Mininprep Kit |
QIAGEN |
Cat#27104 |
| Agilent RNA 6000 Nano Kit |
Agilent |
Cat#5067–1511 |
| Deposited Data |
| RNaseq of H1299 NT RNAi, H1299 PKCi RNAi and H1299 SOX2 RNAi cells |
This paper |
GSE141666 |
| Experimental Models: Cell Lines |
| NCI-H1299 |
ATCC (the American Type Culture Collection) |
CRL-5803; RRID: CVCL_0060 |
| NCI-H520 |
ATCC (the American Type Culture Collection) |
HTB-182; RRID: CVCL_1566 |
| NCI-H2170 |
ATCC (the American Type Culture Collection) |
CRL-5928; RRID: CVCL_1535 |
| A549 |
ATCC (the American Type Culture Collection) |
CCL-185; RRID: CVCL_0023 |
| Experimental Models: Organisms/Strains |
|
Trp53fl/fl mice |
The Jackson Laboratory |
008462 |
| KP mice |
Alan Fields Laboratory |
N/A |
| Oligonucleotides |
| See Table S6
|
|
N/A |
| Recombinant DNA |
| See Table S7
|
|
N/A |
| Software and Algorithms |
| cBioportal for Cancer Genomics |
Memorial Sloan Kettering Cancer Center |
http://www.cbioportal.org/ |
| SigmaPlot |
Systat Software Inc. |
https://systatsoftware.com/products/sigmaplot/ |
| Image-Pro Plus 7.0 |
MEDIA CYBERNETICS |
https://www.mediacy.com/imageproplus |
| Living Image software |
PerkinElmer |
https://www.perkinelmer.com/lab-products-and-services/resources/in-vivo-imaging-software-downloads.html#LivingImage |
| Gene Set Enrichment Analysis (GSEA), hallmark analysis (version h.all.v6.2) |
Broad Institute |
http://software.broadinstitute.org/gsea/index.jsp |
| Ingenuity Pathway Analysis (IPA) |
QIAGEN Bioinformatics |
https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/ |
| Transcription Factor Target Gene Database |
Institute for Systems Bioloy |
http://tfbsdb.systemsbiology.net/ |
| The MEME Suite: Motif-based sequence analysis tools |
National Institutes of Health |
http://meme-suite.org/ |
| GraphPad Prism 7 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
| Aperio ImageScope |
Leica Biosystems |
https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/ |
