Table 1.
Summary description of the experiments
| Experiment | Time point | Parasite isolates (gam/µl) | Measured traits | Mean ± SE (median) [range] number of mosquitoes for each parasite isolate | |
|---|---|---|---|---|---|
| Irradiated | Unirradiated-control | ||||
| Experiment 1: Effects of irradiation on An. arabiensis competence for P. falciparum | 7 dpbm | A (64); C (160); D (88); E (32); G (48); H (56); I (48); J (32) | Oocyst prevalence: the number of mosquitoes harboring at least one oocyst in their midguts out of the total number of dissected mosquitoes |
47.9 ± 4.9 (50.0) [21–71] (n = 383) |
47.3 ± 4.9 (51.0) [23–66] (n = 378) |
| Oocyst intensity: the mean number of oocysts in infected mosquitoes |
19.0 ± 2.7 (19.5) [5–31] (n = 180) |
22.5 ± 3.22 (20.0) [13–37] (n = 152) |
|||
| 14 dpbm | E (32); H (56); I (48); J (32); K (72); L (168); M (32); N (136); O (96); P (96) | Sporozoite prevalence: the number of mosquito head/thorax detected positive to P. falciparum using qPCR out of the total number of dissected heads/thoraces |
47.3 ± 3.7 (50.0) [17–55] (n = 473) |
48.9 ± 4.35 (48.5) [27–78] (n = 489) |
|
| Sporozoite intensity: the mean number of amplification cycle during qPCR (the lower the Cq, the higher the sporozoite intensity) |
25.7 ± 2.7 (26.0) [9–38] (n = 257) |
24.8 ± 4.7 (23.5) [7–59] (n = 248) |
|||
| Experiment 2: Effects of irradiation on An. arabiensis survival | 1–7 dpbm | A (64); C (160); D (88); G (48) | From 1 to 7 dpbm, the number and time of death was recorded among mosquitoes exposed to the infectious blood meal |
41.3 ± 11.0 (35.5) [22–72] (n = 165) |
42.0 ± 8.7 (41.5) [24–61] (n = 168) |
| 1–14 dpbm | E (32); H (56); I (48); J (32); K (72); L (168); M (32); N (136); O (96); P (96) | From 1 to 14 dpbm, the number and time of death was recorded among mosquitoes exposed to the infectious blood meal |
88.0 ± 9.9 (85.5) [31–146] (n = 880) |
80.3 ± 11.9 (62.5) [41–137] (n = 803) |
|
| 1–35 dpbm | J (32) | From 1 dpbm until all mosquitoes had died (35 dpbm), the number and time of death was recorded among both infected mosquitoes and uninfected control mosquitoes | Infected: 26 | Infected: 14 | |
| Uninfected: 49 | Uninfected: 45 | ||||
Abbreviations: n, total sample size; SE, standard error