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. 2020 May 19;11:185. doi: 10.1186/s13287-020-01694-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Morphology and phenotype characteristics of pancreatic β cells derived from hiPSCs at the final maturation stage. A Morphological changes of hiPSCs during differentiation into mature pancreatic β cells. B The pancreatic β cells derived from hiPSCs were stained with DTZ. Scale bars, 500 μm (b1, b2, b3, b4). C Scanning electron microscopy of IPCs derived from hiPSCs. The IPCs have secretory granules and complete capsules. Scale bars, 1 μm (c1); 0.5 μm (c2, c3). D Immunofluorescence staining showing that the differentiated hiPSCs at the final mature stage co-expressed PDX1 and NKX6.1, insulin and glucagon, and PDX1 and insulin (scale bars = 50 μm)