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. 2020 May 19;11:181. doi: 10.1186/s13287-020-01682-y

Fig. 1.

Fig. 1

Effect of serum deprivation on growth kinetics and cell spread area of WJ-MSCs. a Comparison of population doublings between control, serum-deprived, and PI3Kinase inhibitor (LY294002)-treated WJ-MSCs (n = 3). Cultures between passages 4–6 were used. b Representative phase contrast images of WJ-MSCs under control and serum-deprived conditions showing morphology are displayed (n = 3). c Cell area was quantified and plotted for a total of 150 cells from 3 different biological samples and compared. d A representative Gaussian distribution plot from 30 cells is presented to show cell area comparison between control and serum-deprived WJ-MSCs. Serum-deprived condition represented as no serum (NS) in the figure. *p < 0.05, **p < 0.01