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. 2020 May 19;11:181. doi: 10.1186/s13287-020-01682-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Impact of serum deprivation on de-adhesion dynamics and expression levels of VTN and p53. a De-adhesion dynamics of serum-deprived WJ-MSCs. WJ-MSCs were treated with TrypLE and the time taken by the MSCs to contract to a rounded morphology was recorded with the help of time lapse imaging. The normalized area vs. time data were fitted to a Boltzmann sigmoid equation to determine the time constants τ1 and τ2. Delayed de-adhesion leading to greater values for both the time constants were observed. Differences in τ1 and τ2 between control and serum-deprived WJ-MSCs were significant. A total of 45 cells from 3 different biological samples were compared. b mRNA expressions of VTN as detected by qRT-PCR (n = 5). c Band density of protein expression levels of VTN was quantified relative to GAPDH and plotted (n = 3). d Representative images of Western blots. GAPDH was used as a loading control. MCF7 cells were used as negative control (n = 5), as they did not show much increase in expression under serum deprivation. Out of the five sets of experiments performed, two of them showed a small increase in VTN protein expression. e The distribution of VTN (green) and vimentin (red) was visualized by immunofluorescence. Insets show nuclei stained with DAPI. Representative images from four independent biological samples are shown (n = 4). f p53 mRNA was detected by qRT-PCR (n = 4). g Band density of protein expression levels of p53 was quantified relative to GAPDH and plotted (n = 3). Serum-deprived condition represented as no serum (NS) in the figure (*p < 0.05, ***p < 0.0001)