Fig. 3.

Influence of PI3kinase pathway inhibition on cell proliferation, cell cycle status, and viability of WJ-MSCs under serum deprivation. a WJ-MSCs, under serum deprivation, were treated with and without PI3Kinase inhibitor (LY294002) and protein expression levels for VTN and p53 were determined by Western blotting. GAPDH was used to confirm equal loading. Band densities were quantified relative to GAPDH and plotted. Each value represents mean ± SEM of at least four independent biological samples (n ≥ 4). b MTT assay compared the percentage of metabolically viable cells between WJ-MSCs treated with and without LY294002 under serum deprivation (n = 3). c Cell cycle analysis of WJ-MSCs, under control and serum-deprived condition, with and without LY294002 treatment. WJ-MSC populations were treated with propidium iodide and DNA content was analyzed by flow cytometry (n = 3). d Percentages of cells in each phase of the cell cycle are shown by the histograms (n = 3). e Detection of apoptosis in WJ-MSCs treated with and without LY294002 under serum-deprived condition. WJ-MSCs cultured with 10% FBS were used as control. f Comparison of the percentages of viable, early apoptotic, and late apoptotic populations between control and serum-deprived WJ-MSC cultures in the presence and absence of LY294002 as depicted by the histogram. Each bar represents mean ± SEM. Data shown are representative of at least three independent biological samples (푛n=4). g Detection of apoptosis in control and serum-deprived MCF7 cells. h Comparison of the percentages of viable, early apoptotic, and late apoptotic populations between control and serum-deprived MCF7 cultures. Data shown are representative of two independent experiments. Each bar represents mean. Serum-deprived condition represented as no serum (NS) in the figure (*p < 0.05, **p < 0.01, ***p < 0.0001)