Fig. 5.

Liraglutide reduces apoptosis of hCMSCs via PKA/β-catenin pathway. a Western blot and b RT-qPCR verify the knockdown effects of three Si-GLP-1R in hCMSCs. c, d Western blot was used to detect of β-catenin and p-β-catenin expression under the stimulation of LPS by adding 20 μM H89 or 100 nM Si-GLP-1R and liraglutide. e The expression of apoptotic proteins Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 was detected by western blot with PKA inhibitor H89 and liraglutide. f The expression of GLP-1R and apoptotic proteins Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were detected by western blot with Si-GLP-1R and liraglutide. Error bars represent mean ± SD from three independent experiments. Compared with Si-con group, ***P < 0.001