Table 3.

Daily scoresheet for assessment of neurotoxicity or signs of injury at the doxorubicin injection site. The maximum reachable score was 7. At a score of 1 or higher, the mouse concerned was euthanized. A score of 0 for any given parameter indicates ‘no’, and a score of 1 indicates ‘yes’.

Parameter	Score	In case of
Mouse grimace scale	≤1 (score 0)/ >1 (score 1)
Shaking	0/1
Head tilt	0/1
Manage movements	0/1
Exophthalmus	0/1	Retrobulbar sinus injection
Enophthalmus	0/1	Retrobulbar sinus injection
Eyelid closure	0/1	Retrobulbar sinus injection
Increase in tail size	0/1	Lateral tail vein injection
Skin lesions	0/1	Lateral tail vein injection
Reduced tail movement	0/1	Lateral tail vein injection