Figure 2.

Messenger RNA concentrations (fg mRNA per 50 ng total RNA) and protein levels (Densiometric Units; D.U.) for Adiponectin (a, b) and DIO2 (c, d) in perirenal fat from 14-day old control (CONT) lambs gestated and delivered under normoxic conditions, and 14-day old lambs exposed to long term moderate gestational hypoxia in utero (LTH) then maintained post-birth at either high altitude (HA) or near sea level (SL) through 14 days post-birth. Lambs exposed to LTH in utero had reduced mRNA and protein compared to CONT lambs in the PRF. However, for mRNA, only the LTH-SL lambs did this reach significance. For both LTH cohorts, however, protein was significantly reduced. For DIO2, both LTH-SL and LTH-HA groups exhibited significantly reduced mRNA, while for protein, a reduction was only noted in the LTH-HA group. (lanes 1–3 CONT; Lanes 4–6 LTH-SL; Lanes 7–9 LTH-HA)