Figure 4.

miR160-OE plants are auxin hypersensitive and miR160-KD plants are hyposensitive. (a) Roots of plants grown without or with (0.3 or 1.0 μM) auxin (2,4-D) for 12 days. (b) Quantification of root length after exogenous auxin treatment. Boxplots were prepared using data from 12 individual plants in each treatment. Grey-filled circles are data from individual plants and black-filled triangle is the mean of the data points. Drastic reduction in root length in miR160-OE-1 plants at 0.3 μM auxin treatment shows that miR160-OE plants are hypersensitive to auxin. Statistically significant differences were estimated using one-way ANOVA followed by a Tukey HSD test (p < .05). (c) Percentage reduction in root length in 1.0 μM treatment compared to untreated control shows that miR160-KD-1 plants are hyposensitive to auxin. This experiment was performed twice with similar results.