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. 2020 Apr 7;15(5):1746034. doi: 10.1080/15592324.2020.1746034

Figure 6.

Figure 6.

Annotation of unigenes, functions and validation of DEGs (a) Venn diagram of the number of unigenes annotated in four public databases. (b) Volcano plot of DEGs. The x-axis represents the log2-transformed fold change; the y-axis represents the -log10 false discovery rate, red points represent upregulated DEGs, green points represent downregulated DEGs, and black points represent non-DEGs. (c) Correlation of gene expression results obtained using RNA-Seq and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Correlation assay performed for 18 DEGs with a log2 ratio of 21.00 or S-1.00. FDR, false discovery rate; FC, fold change. (d) Gene Ontology (GO) enrichment classification of differentially expressed genes (DEGs). (e) Pathway functional enrichment of DEGs. The x axis represents the enrichment factor, the y axis represents the pathway name, and the color indicates the q-value, with a lower q-value indicating more significant enrichment and the point size indicating the DEG number.