Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Cell Physiol. 2020 Jan 10;235(6):5318–5327. doi: 10.1002/jcp.29419

Figure 3: RUNX1 and ts-112 have reciprocal expression trends in breast cancer cell lines.

Figure 3:

Expression of RUNX1-responsive candidates (a) ts-19, (b) ts-29, (c) ts-46, and (d) ts-112 in MCF10A normal-like mammary epithelial cells and four breast cancer cell lines (MCF7, MCF10AT1, MCF10CA1a, MDA-MB-231). Biological replicate mean (n = 3) and SEM graphed; ANOVA significance. Microarray tsRNA expression data from (Balatti et al., 2017). (e) qPCR validation of ts-112 expression normalized to U6 and average expression in MCF10A biological replicates (n = 3). Mean expression and SEM plotted with unpaired two-tailed t-test significance. (f) qPCR expression difference of RUNX1 (gray - downregulated) and ts-112 (black - upregulated) in four breast cancer cell lines as compared to MCF10A normal-like mammary epithelial cells. Biological replicate mean (n = 3) with Dunnett’s multiple comparisons test significance. RUNX1 qPCR normalized to GAPDH and HPRT. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.