Table 1:
Differentially expressed of tsRNA following RUNX1 inhibition in MCF10A cells
| Comparison | p < 0.05 | ≥ 2-fold | ≥ 2-fold p < 0.05 |
≥ 1.5-fold | ≥ 1.5-fold p < 0.05 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Up | Down | Up | Down | Up | Down | Up | Down | |||
| shNS (Control) -> shRUNX1-C1 | 43 | 3 | 3 | 3 | 3 | 17 | 12 | 15 | 10 | |
| shNS (Control) -> shRUNX1-C4 | 24 | 2 | 5 | 1 | 3 | 15 | 13 | 8 | 7 | |
| shNS -> both shRUNX1-C1 & -C4 | 21 | 2 | 2 | 1 | 2 | 9 | 8 | 5 | 6 | |
| Identity of tsRNA differentially expressed in both shRUNX1-C1 and shRUNX1-C4 as compared to shNS control | ts-107 ts-52 | ts-46 | ts-19 | ts-46 | ts-19 | ts-41 | ts-100 | ts-43 | ts-14 | |
| ts-14 | ts-54 | ts-65 | ts-29 | ts-29 | ts-43 | ts-104 | ts-46 | ts-19 | ||
| ts-17 | ts-55 | ts-46 | ts-14 | ts-54 | ts-29 | |||||
| ts-18 | ts-56 | ts-54 | ts-19 | ts-56 | ts-48 | |||||
| ts-19 | ts-58 | ts-56 | ts-29 | ts-58 | ts-55 | |||||
| ts-29 | ts-62 | ts-58 | ts-48 | ts-99 | ||||||
| ts-34 | ts-72 | ts-65 | ts-55 | |||||||
| ts-4 | ts-76 | ts-7 | ts-66 | |||||||
| ts-43 | ts-8 | ts-99 | ||||||||
| ts-46 | ts-99 | |||||||||
| ts-48 | ||||||||||
Note: Comparison is fold change and one-way ANOVA between non-silencing (shNS) control and RUNX1 inhibition with two different shRNA (-C1, -C4) in MCF10A normal-like mammary epithelial cells.