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. 2020 Apr 23;9(4):547. doi: 10.3390/plants9040547

Figure 1.

Figure 1

Phenotype of osbhlh073-D plants and the expression level of OsbHLH073. (A) Schematic diagram of the OsbHLH073 gene structure and relative T-DNA insertion positions. Black boxes denote exons. 4XE denotes four tandem copies of CaMV 35S enhancer. Primers (a, b, and c) used for genotyping. (B) Genotyping analysis of osbhlh073-D plants. WT, Ht, and Hm mean wild-type segregant, heterozygote, and homozygote for T-DNA insertion, respectively. (C) Phenotype of wild-type, heterozygous, and homozygous plants. Bar = 20 cm. (D) Panicle exsertion phenotype of wild-type and osbhlh073-D (073-D) plants. Bar = 5 cm. (E) Photograph showing internode length of wild-type and osbhlh073-D (073-D) plants. Bar = 20 cm. (F) Average internode length of wild-type and osbhlh073-D plants. Data are mean ± standard deviation (SD) from at least 10 plants. (* p < 0.001, Student’s t-test) (G) Relative expression level of OsbHLH073 in wild-type, heterozygous, and homozygous plants. Expression levels were normalized to Actin (LOC_Os03g50885) (** p < 0.01, *** p < 0.001, Student’s t-test).