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. 2020 May 18;11(1):414–429. doi: 10.1080/21505594.2020.1763061

Figure 2.

Figure 2.

Confirmation of functional presence of the CD73 and the effect of P. gingivalis infection on CD73 activity in GECs. GECs were incubated in phosphate-free buffer and treated with either AMP, APCP, and/or P. gingivalis. All graphs (a-c) were obtained using Malachite Green Phosphate assay kit following the manufacturer’s instructions (Bioassay Systems). a) Inhibition–concentration curve showing CD73 activity of cells pre-treated with AMP [100 μM] and further incubated with varying concentrations of APCP. b) CD73 activity in untreated and P. gingivalis infected GECs (1, 6, and 24 h) in the presence of AMP [100 µM] and/or APCP [10 µM] N = 6, *p < 0.05 as compared for AMP vs. AMP + APCP. Mean ± SEM. c) CD73 activity with/without P. gingivalis infection at varying AMP concentrations. Cells were infected with P. gingivalis for 6 h and stimulated with AMP for 30 min post infection. N = 6, *p < 0.05 as compared to AMP 0 μM; Mean ± SEM.