Figure 7.

CD73 overexpression reverses the decreasing P. gingivalis intracellular level in GECs caused by exogenous treatment of human IL-6. a) Cells were incubated with multiple doses of IL-6 for 24 h. GEC cell viability was assessed using MTT assay. n.s = no statistically significant difference, N = 3, * p < 0.05. For B and C, GECs were infected with P. gingivalis (MOI 100) for 6 h. b) Human recombinant active IL-6 [25 ng/mL] was added to cells 30 min prior to infection at varying concentrations. IL-6 treatment [25 ng/mL] was performed similarly in B and C. c) Overexpression of CD73 (OE) was implemented in some conditions as described in Material & Methods. For B and C, cells were later incubated with metronidazole [200 μg/mL] and gentamicin [300 μg/mL] for an additional 1 h to kill extracellular bacteria. RNAs from each sample were isolated using Trizol and subsequently obtained cDNAs were subjected to qPCR analysis using P. gingivalis 16s rRNA primers. N = 6, *p < 0.05, **p < 0.001, *** <0.0001; Mean ± SEM. CD73OE = CD73 overexpression, NAC = N-acetyl-l-cysteine (ROS scavenger; 50 µM).