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[Preprint]. 2020 Mar 20:2020.03.18.997585. [Version 1] doi: 10.1101/2020.03.18.997585

Fig. 2. Incorporation of 2’-F,Me-UTP, 3’-F-dTTP, TFV-DP and 3’-N3-dTTP by SARS-CoV-2 RdRp to terminate the polymerase reaction.

Fig. 2

The sequences of the primer and template used for these extension reactions, which are at the 3’ end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2’-F,Me-UTP, (b) 3’-F-dTTP, (c) UTP + TFV-DP, and (d) 3’-N3-dTTP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8), the indicated RNA template and primer, and the appropriate reaction buffer, followed by detection of reaction products by MALDI-TOF MS. The detailed procedure is shown in the Methods section. The accuracy for m/z determination is ± 10 Da.