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. 2020 May 13;11:420. doi: 10.3389/fpls.2020.00420

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Copyright © 2020 Zhang, Chen, Du, Zhang, Devkota, Chen, Chen, Sun and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adaxial and abaxial epicuticular waxes had different contribution to leaf transpiration barrier. -Ad, adaxial epicuticular waxes were removed by gum Arabic; -Ab, abaxial epicuticular waxes were removed by gum Arabic; -Ad/-Ab, epicuticular waxes from both surfaces were removed by gum arabic. Data are expressed as means ± standard error (n = 5). Statistical analysis was performed among different treatments at same time point, and different letters at same time point indicate statistically significant (p < 0.05). (A) Leaf morphological changes after epicuticular wax removal by gum arabic; (B) Leaf water loss was affected by the epicuticular wax removal.