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Overview of the microfluidic platform for molecular analysis of cell protrusions. A) Schematic illustration of invasive cells in a migrating tumor front. B) The generation of uniform cell-protrusion arrays in microfluidic chips, which provide an in vitro model for cancer cell migration. C,D) After removing the microfluidic mold and fixing, selected cell protrusions are cut using LCM at single-cell precision, collected on the surface of LCM caps, and lysed for biomolecular analysis.
