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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: Immunity. 2019 Jul 2;51(1):90–103.e3. doi: 10.1016/j.immuni.2019.06.003

Figure 1. 3-D imaging approach provides accurate tissue volume and cell localization.

Figure 1.

(A) 3-D images of scanned volume and processed tissue volume in ileum. (B) 3-D reconstruction of villi and crypts from full-thickness BALB/c ileum; red, CD3+ T cells; white, nuclei. Note that villus tissue volume is larger than crypt tissue volume: mean villus Z-depth is approximately 250 μm; mean crypt Z-depth is approximately 100 μm. (C) Quantification of CD3+ T cell number per full-thickness 3-D field in BALB/c ileum. (D) Quantification of CD3+ T cell density in BALB/c ileum per full-thickness 3-D field; n = 17 (villus region) and n = 16 (crypt region) independent 3-D views (with 4–5 independent 3-D views per mouse and 4 mice per group). Bar graphs represent mean and SEM; **p < 0.01. Data combined from two independent experiments.