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The sequence of the designed primers used for Real-time polymerase chain reaction
| Annealing temperature(°C) | Size (bp) |
Primer sequences (5′–3′) | Gene |
|---|---|---|---|
| 55.91 57.63 |
131 | F5 AAAGCAACAAGTTCCCCAG 3´ R 5´ CCCACTGCTCAGGTCACAC 3´ |
BDNF |
| 58.34 57.74 |
222 | F 5´AAGGTCATCCCAGAGCTGAA3´ R 5 ´ CCCACTGCTCAGGTCACAC3´ |
GAPDH |
BDNF: brain-derived neurotrophic factor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase
