Figure 4.

Ovarian Mfn2 mRNA expression, Mfn2, Bcl2 and Bax protein levels

A) Mfn2 mRNA expression of different experimental groups. B) The most representative image of western blotting of ovarian Mfn2 protein level of different experimental groups. C) Mfn2 protein level in different experimental groups. D) The most representative image of western blotting of ovarian Bcl2 and Bax protein levels of different experimental groups. E) Bcl2 protein level in different experimental groups. F) Bax protein level in different experimental groups. G) Comparison of ovarian Mfn2, Bcl-2, and Bax protein levels in different experimental groups. The data represent means±SD (n=10/group). Our data showed that cisplatin significantly decreased Mfn2 (P<0.001), and Bcl2 mRNA expressions and protein concentrations (P<0.001) and significantly increased Bax mRNA expression and protein concentration (P<0.001) compared to control group. RJ restored Mfn2 mRNA expression and protein. Added to that, RJ re-established ovarian Bcl2/ Bax mRNA and proteins (P<0.001) compared to cisplatin group. a: indicates a significant difference when compared to the control group at P˂0.05, b: indicates a significant difference when compared to cisplatin group at P<0.05. (Mfn2): Mitofusin 2, (RJ): Royal jelly