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. 2020 May 20;9:e54469. doi: 10.7554/eLife.54469

Figure 3. Signaling by WT RSPO3 and RSPO3 ΔTSP/BR HS20 causes internalization and degradation of RNF43 in the presence and absence of LGRs.

Figure 3.

Cell-surface RNF43-Flag (captured by cell-surface biotinylation; see Materials and methods), total RNF43-Flag and total actin were visualized by immunoblotting after treatment of WT HAP1-7TGP (A) or LGR4/5/6KO (B) cells with the indicated RSPO3 variants.