Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6 | |||
| Genetic reagent (Mus musculus) | VAMP2 – VAMP3 null allele | Borisovska et al., 2005 | PMID:15920476 | |
| Antibody | mouse anti- VAMP2 |
Synaptic Systems | Cat# 104 211 | ICC, 1:1000 |
| Antibody | rabbit anti-VAMP3 | Synaptic Systems | Cat# 104 103 | ICC, 1:1000 |
| Antibody | Alexa Fluor 555 goat anti-mouse | Invitrogen | Cat# A21422 | ICC: 1:1000 |
| Antibody | Alexa Fluor 488 goat anti-rabbit | Invitrogen | Cat# A11008 | ICC: 1:1000 |
| Recombinant DNA reagent (Mus musculus) | VAMP2 | GeneID: 22318 | cDNA (Mus musculus) | |
| Recombinant DNA reagent (Mus musculus) | VAMP1 | GeneID: 22317 | cDNA (Mus musculus) | |
| Recombinant DNA reagent (Mus musculus) | VAMP8 | GeneID: 22320 | cDNA (Mus musculus) | |
| Recombinant DNA reagent (Mus musculus) | CpxII | GenBank: U35101.1 | cDNA (Mus musculus) | |
| Transfected construct (Mus musculus) | pSFV-VAMP2- IRES-EGFP |
this paper | Semliki Forest virus derived from 22318, expression of wild type full length protein | |
| Transfected construct (Mus musculus) | pSFV-VAMP1- IRES-EGFP |
this paper | Semliki Forest virus derived from 22317, expression of wild type full length protein | |
| Transfected construct (Mus musculus) | pSFV-VAMP8- IRES-EGFP |
this paper | Semliki Forest virus derived from 22320, expression of wild type full length protein | |
| Transfected construct (Mus musculus) | pSFV-VAMP2-poly L-IRES-EGFP | this paper | Semliki Forest virus derived from 22318 with indicated mutations |
|
| Transfected construct (Mus musculus) | pSFV-VAMP2-VAMP1TMD-IRES-EGFP | this paper | Semliki Forest virus derived from 22318 and from 22317, chimeric mutant protein | |
| Transfected construct (Mus musculus) | pSFV-VAMP2-VAMP8TMD-IRES-EGFP | this paper | Semliki Forest virus derived from 22318 and from 22320, chimeric mutant protein | |
| Transfected construct (Mus musculus) | pSFV-CpxII- IRES-EGFP |
this paper | Semliki Forest virus derived from U35101.1; expression of wild type full length protein |
|
| Software algorithm | IgorPro | WaveMetrics Software | ||
| Software algorithm | AutesP | NPI electronics | ||
| Software algorithm | ImageJ | NIH | ||
| Software algorithm | Zen2008 | Zeiss |