Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 14;8(1):e000610. doi: 10.1136/jitc-2020-000610

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

The effect of inhibiting CD39, CD73, and A2AR in vivo in the 5T33MM model. (A) Schematic picture for the experiment (mpk=mg/kg). (B) FACS analysis of CD39 expression on CD138⁺ 5T33 MM cells. (C–G) C57Bl6KalwRij mice were treated with either a CD39 inhibitor (POM-1), CD73 antibody, and/or an A2AR inhibitor as described in the Methods section. Spleens were weighed (C) and cells from five of the mice were further analyzed for CD138⁺ MM cells by FACS analysis, CD138⁺ cells/spleen were calculated (n=5) (D). BM was collected for analysis of BM plasmacytosis on cytosmears (E) and serum was collected for M-protein analysis by protein electrophoresis (F). Analysis of spleen cells by FACS as described shows the ratio of CD3⁺ cells per number of CD138⁺ tumor cells in the spleen (n=5) (G). Results are expressed as mean and SD. Statistical differences were calculated using one-way Analysis of variance (ANOVA). A2AR, adenosinereceptor A2A; BM, bone marrow; MM, multiple myeloma.