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. 2020 Apr 15;6(16):eaay9035. doi: 10.1126/sciadv.aay9035

Fig. 3. In vitro macrophage uptake of nanoparticles.

Fig. 3

(A) Fluorescent nanoparticles were incubated with RAW 264.7 macrophages for 4 hours, and uptake was analyzed by flow cytometry. Relative uptake, as measured by geometric mean fluorescence normalized to untreated cells, was significantly reduced as a result of anisotropy and membrane coating. (B) Macrophage uptake of nanoparticles (pink) was visualized by confocal imaging and found to be reduced for anisotropic coated particles. Macrophages were stained for nuclei (blue) and actin (green). Scale bars, 20 μm. Data are shown as means ± SEM (n = 4 replicates). Statistics were performed by a two-way ANOVA with Bonferroni’s posttests (*P < 0.05 and ***P < 0.001).