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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Neurochem. 2019 Dec 29;153(4):468–484. doi: 10.1111/jnc.14938

Figure 2.

Figure 2

Nicotine potentiates network-wide spiking and bursting, and reorganizes network activity in a concentration-dependent manner. (a-c) MEAs treated with nicotine concentrations of 50 μM and greater show a significant increase in (a) spikes, (b) bursts and (c) spikes within bursts during a 3-minute recording. Data expressed as percent change normalized to baseline. (n=5 MEAs for each treatment from two independent cultures; *p<0.05, **p<0.01, ****p<0.0001; one-way ANOVA and Dunnett post hoc correction). (d-f) Representative spatial heat maps of correlation coefficients between active electrodes of the network (d) during baseline, (e) 1 minute, and (f) 15 minutes after applying 50 μM nicotine. (g) Quantification of correlation coefficients between active electrodes of the network after 1 and 15-minute application of 50 μM nicotine. (n=5 MEAs for each treatment; *p<0.05, ns = not significant; repeated-measures ANOVA with Tukey’s post hoc correction). Error bars represent SEM. (h-j) Representative spatial heat maps of correlation coefficients between active electrodes of the network (h) during baseline, (i) 1 minute, and (j) 15 minutes after applying 90 μM nicotine. (k) Quantification of correlation coefficients between active electrodes of the network after 1 and 15-minute application of 90 μM nicotine. (n=5 MEAs for each treatment from two independent cultures; **p<0.01, ns = not significant; repeated-measures ANOVA with Tukey’s post hoc correction). Error bars represent SEM.