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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Neurochem. 2019 Dec 29;153(4):468–484. doi: 10.1111/jnc.14938

Figure 6.

Figure 6

Nicotine induces NMDAR-dependent CaMKII phosphorylation in primary cultured hippocampal neurons. (a,d) Neurons (DIV14) plated on tissue culture dishes were pre-incubated with vehicle (a) or APV (50 μM, 15 min) (d) and subsequently treated with nicotine (90 μM for 1, 5, or 15 min). Lysates were immunoblotted for CaMKII-α/β phosphothreonine 286 (pT286) and total CaMKIIα as a loading control. (b-c, e-f) Quantification of average intensity of CaMKIIα/β pT286. (n=3 independent wells from a single culture for each condition; *p < 0.05, **p < 0.01; ns = not significant; one-way ANOVA with Holm-Sidak post-hoc correction). Error bars represent SEM. Full uncropped blots are shown in Supplemental Figure S7.