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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Curr Biol. 2020 Apr 2;30(10):1958–1964.e3. doi: 10.1016/j.cub.2020.03.013

Figure 4. MT function during intercalation.

Figure 4.

A-B, Side projections of intercalating MCCs expressing GFP-EB3 (A) or GFP-CAMSAP2 (B) and RFP-Sas6 stained with phalloidin and DAPI. C, Side projection of intercalating control and GFP-CAMSAP1-Par6 ICs stained with α-acetyl. tub. and phalloidin. D, Quantification of acetyl. tub. in control and GFP-CAMSAP1-Par6 ICs. Fluorescence was normalized relative to the control (uninjected) IC in mosaic embryos for each experiment. E, Side projection of embryos injected with EGFP-ninein to mark MT (−) ends and Pen-RFP or RFP-CAMSAP1-Par6 and stained with phalloidin and DAPI. F, Embryos mosaically injected with Pen-RFP and GFP-CAMSAP1-Par6 mRNA and stained with phalloidin to assay apical insertion. G, Quantification of the percentage of ICs apically inserted at each stage. For all bar graphs, bars represent the average, error bars indicate SD, and *p<0.05, **p<0.01. Scale bars in A–D are 5μm and in E is 10μm. The n’s for each experiment are indicated in Table S1. See also Figure S4 and Table S1.