Fig. 2.
Coordination environment of WT and L61G variant BaR2b metal sites in crystals prepared without oxygen (a, b) and aerobically (c, d). The site of the mutation is marked with an asterisk. a Metal site of WT BaR2b crystal soaked with MnII and FeII in anoxic (green) and aerobic conditions (pink). b Overlay of the L61G (yellow) and WT R2b (transparent green, as in a) metal sites from crystals soaked with equal amount of MnII and FeII in anoxic conditions. c Overlay of the anaerobically prepared L61G (transparent yellow, as in b) and L61G R2b (orange), prepared in aerobic conditions. Crystals were soaked with equal amount of MnII and FeII. d Overlay of the metal sites in WT (purple) and L61G (blue) apo R2b. Metal ions are shown as large spheres, solvent molecules—as small spheres. Solid lines indicate hydrogen and metal coordinations, while dashed lines indicate metal–metal distances (in Å)