Fig. 4.

Relative amounts of Mn and Fe in the two metal sites in a WT and b L61G variant BaR2b obtained when reconstituting the cofactor in absence and presence of oxygen. The relative amounts (shown in orange for Fe and purple for Mn) are derived from the integrated intensity of the anomalous difference density peaks at the Fe and Mn absorption edges. Individual measurements (n number of metal sites) are depicted as cyan circles and blue triangles. The values for each measurement are given in Supplementary Table 1