Fig. 4.

Impact of hemin on inactivation induced by DPP6a. a Representative inside-out current recordings from HEK293t cells expressing Kv4.2 alone (left) or together with human DPP6a (center) or its mutant C13S (right). Currents were measured at 60 mV before (black) and 200 s after application of 200 nM hemin in the presence of 200 μM reduced GSH (red). The green trace in the center panel is the trace with hemin but scaled in amplitude to match the peak current of the control. The N-terminal sequence of human DPP6a is shown at the top. b Time course of the peak currents for the indicated constructs and hemin application. c Time constants of fast inactivation, normalized to the control values before hemin application. Symbol use and n as in (b). Data in (b) and (c) are means ± SEM with n indicated in (b)