Fig. 2.

Mass transfer of permeation marker LY (AUC values, 15–60 min) over artificial membranes subjected to varying stages of LBF digestion (inhibited enzymes). Negative controls comprised LY in digestion medium (FaSSIF) or lipolysis buffer (used as control for significance testing). Positive controls comprised LY in FaSSIF on either naked polycarbonate filters or GIT-0 membranes disrupted by applying the solution to pre-wet filters. Values above the dashed line indicates a major loss of membrane integrity. (a) Hexadecane membranes (HDM), (b) GIT-0 membranes (soy lecithin in n-dodecane) with phosphate buffer (PB, 10 mM) in the receiver compartment, (c) GIT-0 membranes with Acceptor Sink Buffer (containing 20 mM HEPES and 1% w/v SDS) in receiver compartment, and (d) GIT-0 membranes with PB supplemented with 4% (w/w) bovine serum albumin (BSA) in receiver compartment.