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. 2020 May 14;8:413. doi: 10.3389/fbioe.2020.00413

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Copyright © 2020 Ibáñez-Fonseca, Santiago Maniega, Gorbenko del Blanco, Catalán Bernardos, Vega Castrillo, Álvarez Barcia, Alonso, Aguado and Rodríguez-Cabello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images and quantification for collagen staining with Picrosirius red. (A) Polarized light images from muscle samples of empty (left), chemical (center), and physical (right) groups for the scaffold (area without newly formed myofibers) and interface (area with newly formed myofibers) regions at 2 (top) and 5 weeks (bottom) post-injury. Scale bar = 100 μm. (B,C) Percentage of collagen in the interface (including no injury group at 5 weeks) and scaffold regions, normalized to their areas, after 2 and 5 weeks. *p < 0.05, ***p < 0.001.