Figure 1.

Pro-inflammatory stimuli suppress PER2^LUC rhythms in macrophages. (A) Raw PER2^LUC traces of synchronized mPer2^Luc BMDMs stimulated with mock, IFN-γ, TNF-α, or P3C (50 ng/mL for 24 h), and amplitude and period analysis of rhythms from traces. (B) PER2^LUC traces of synchronized BMDMs stimulated with mock, IFN-γ plus TNF-α, IFN-γ plus LPS, IFN-γ plus P3C, or LPS plus P3C (50 ng/mL each for 24 h), and amplitude and period analysis of rhythms. (C) PER2^LUC traces of synchronized peritoneal macrophages stimulated with mock, or IFN-γ plus LPS, and amplitude and period analysis of rhythms. Data are represented as mean ± SE (n = 3). P-values are calculated from one-way ANOVA with post-hoc Tukey tests and are considered to be significantly different with *P < 0.05, **P < 0.005, or ***P < 0.0005.