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. 2020 May 14;11:867. doi: 10.3389/fimmu.2020.00867

Figure 2.

Figure 2

Pro-inflammatory stimuli affect Per2 and Nr1d1 mRNA rhythms. qRT-PCR data showing (A) Per2 mRNA expression over a 32 h circadian time course (2 h resolution) from mPer2Luc BMDMs or mPer1/2 BMDMs. Cells were stimulated with mock or IFN-γ plus TNF-α (50 ng/mL each for 24 h). (B) Per2 mRNA expression over a 30 h circadian time course (3 h resolution) from mPer2Luc BMDMs. Cells were stimulated with mock or IFN-γ plus LPS (50 ng/mL each for 24 h). (C) Nr1d1 mRNA expression in exact conditions as in (A). (D) Nr1d1 mRNA expression in exact conditions as in (B). Genes here were normalized to non-rhythmic Eif2a expression. Data are represented as mean ± SE (n = 3). P–values (*) are calculated from Cosinor analysis and significance (at P < 0.05) is considered to be rhythmic circadianly.