Figure 5.

Anti-inflammatory stimuli enhance PER2^LUC rhythms in macrophages. (A) Detrended PER2^LUC traces of synchronized mPer2^Luc BMDMs stimulated with mock, IL-4, IL-10, IL-13, or dexamethasone (Dex), and amplitude and period analysis of rhythms from traces. (B) Per2 mRNA expression over a 32-h circadian time course (2 h resolution) from mPer2^Luc BMDMs. Cells were stimulated with mock or IL-4. (C) Nr1d1 mRNA expression from cells stimulated with mock or IL-4. Data are represented as mean ± SE (n = 3). In (A), P-values are calculated from one-way ANOVA with post-hoc Tukey tests and are considered to be significantly different with *P < 0.05, **P < 0.005, or ***P < 0.0005. In (B,C), P–values (*) are calculated from Cosinor analysis and significance (P < 0.05) is considered to be rhythmic circadianly.