FIGURE 5.

hrSAA1 does not induce ROS production in CD14⁺ monocytes. Freshly isolated monocytes were stimulated with homogenous rSAA1 (hrSAA1) (10–1000 ng/ml, 0.8–80 nM) in parallel with rSAA1 (10–1000 ng/ml, 0.8–80 nM). Following an incubation period of 1 h, staining with 2′,7′-dichlorofluorescein diacetate (50 μM) was carried out and flow cytometry was used to quantify ROS production. Results are presented as the mean percent change in fluorescence intensity in comparison to buffer-treated cells + SEM (i.e., 0% is equivalent to the same fluorescence intensity as obtained for buffer-treated cells) and are derived from four to seven independent experiments. Significant upregulation in comparison to control is indicated by asterisks (Mann-Whitney U-test; ***p-value < 0.001).