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. 2020 May 14;11:553. doi: 10.3389/fphar.2020.00553

Figure 4.

Figure 4

The HTR2A/PPARγ2 signaling pathway was involved in lipogenesis and the increased expression of inflammatory gene. (A) The messenger RNA (mRNA) expression of Pparγ, Pparγ1, and Pparγ2 in the liver of SD rats. (B) The mRNA expression of Srebp1c in the liver of SD rats. (C) The mRNA expression of Fas in liver of SD rats (D) The protein expression of PPARγ2 in BRL-3A and liver of SD rats. The mRNA expression of Plin2 (E) and Cd36 (F) in BRL-3A. (G) Oil red O staining and lipid content in BRL-3A cells (×400 magnification). (H) The content of triglyceride (TG) in BRL-3A. (I) The mRNA expression of genes related to lipogenesis (Fas, Cd36, and Plin2) in BRL-3A. (J) The mRNA expression of genes related to the inflammatory response (Tnfα, Il6, and Mcp1) in BRL-3A cells. All data are presented as the mean ± SEM (n = 6). ##P < 0.01 and ###P < 0.001, compared with the corresponding control group; *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the corresponding model group; &&P < 0.01 and &&&P < 0.001, compared with the 5-HT+M group. ns, represent no significance.