FIGURE 2.

Schematic representation of aptamer selection for RANKL using SELEX strategy. The process of screening aptamers for RANKL by SELEX includes positive selection and negative selection. A DNA library is incubated with target molecules, RANKL, in the positive selection. The bound sequences are eluted and collected, prior to be incubated with non-target cells. The bound sequences are removed and discarded in the counter selection. While the unbound sequences are amplified and used to initiate the next round of SELEX. The cycle is repeated several times, before the pool is sequenced and characterized.