Figure 4.

Effects of Multiple i.p. Injections of Vγ9Vδ2 T Cells Electroporated with NKG2D CAR (NKG2Dz) on Disease Progression in Tumor-Bearing Mice

(A) Experimental outline of the animal study. NSG mice (n = 6 per group) were i.p. injected with SKOV3-Luc human ovarian cancer cells (1 × 10⁷ per mouse). The treatment started 7 days after tumor cell inoculation, twice a week for 3, 5, and 7 weeks, 1 × 10⁷ CAR-modified Vγ9Vδ2 T cells (γδ T) per injection. PBS injection, twice a week for 3 weeks, was included as a control. (B) Bioluminescent images prior to treatment (day 7) and following the treatments on days 28 and 42. (C) Tumor burden over time by bioluminescent imaging after multiple i.p. injections of CAR-modified Vγ9Vδ2 T cells. Each mouse is represented by one line. (D) Kaplan-Meier analysis of survival. Statistical analysis of survival between groups was performed using the log-rank test. ∗p < 0.05; ∗∗p < 0.01.