Figure 5.

GAA in Microglia and Astrocytes

(A) Microglia (F4/80, left panel); anti-GAA and Hoechst staining show that around half of microglia are GAA positive (arrowheads) and show the presence of GAA-negative resident microglia (arrows); original magnification, ×20. (B) Virtually all astrocytes appeared to contain GAA protein; two representative examples are shown here. The merged panel of the GFAP and GAA staining demonstrates the localization of GAA; original magnification, ×100. Figure S2 shows the reverse magnifications for microglia and astrocytes, respectively.