Figure 7.

Clearance of Glycogen in Articular Cartilage after Gene Therapy Treatment

PAS staining (dark purple granules) of articular cartilage shows glycogen storage in approximately 93% of the chondrocytes of 10-months-old GAA-deficient (GFP) mice. The GAAco (treated mice (GAAco, bottom panel) show a reduction in the number of PAS-positive chondrocytes to approximately 45%, as well as in the intensity of the PAS staining. Wild-type mice (WT, top panel) have no glycogen storage detetctable by PAS staining. Original magnification, ×40; scale bars represent 100 μm.